LOCUS pNLex(NLS) 10174 bp DNA circular 5-SEP-2003 SOURCE ORGANISM COMMENT Encodes LexA-NLS. Predicted pI = 8.99, MW=26,754.8 COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|200319280| COMMENT VNTDBDATE|312926264| COMMENT VNTNAME|pNLex(NLS)| COMMENT VNTAUTHORNAME|Finley| FEATURES Location/Qualifiers promoter 67..1511 /vntifkey="29" /label=ADH1\promoter /note="ADH1 promoter" CDS 1538..2143 /vntifkey="4" /label=LexA /note="LexA DBD (amino acids 1-202)" primer_bind 2105..2143 /vntifkey="28" /label=5'\RF126 /note="homologous to 5' end of RF126 primer" primer_bind 2126..2143 /vntifkey="28" /label=3'\3KF-BD /note="homologous to 3' end of 3KF-BD primer" primer 1776..1796 /vntifkey="27" /label=LexA\Central-s /note="LexA CDS primer, located in centre of DBD ORF" primer_bind 1541..1565 /vntifkey="28" /label=3'\RFx129 /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA" primer 2117..2143 /vntifkey="27" /label=LexA\3' /note="sense primer for PCR amplification of LexA DBD plasmid inserts" primer 2119..2139 /vntifkey="27" /label=JZ45 /note="hybridize to the very end of LexA" primer_bind 1878..1902 /vntifkey="28" /label=LexA-3PR /note="primer for nested PCR in LexA-CS products, also for sequencing" CDS 2150..2173 /vntifkey="4" /label=NLS /note="Nuclear Localisation Sequence" CDS complement(6122..6781) /vntifkey="4" /label=HIS3 /note="HIS3 gene, location according to Genbank file" CDS 8139..8999 /vntifkey="4" /label=ampR /note="ampicillin resistance gene" rep_origin <2898..>4794 /vntifkey="33" /label=2\µm\origin /note="2µm origin, the part that matches part of YEP24" terminator 2217..2547 /vntifkey="43" /label=ADH\T /note="ADH Terminator region" primer_bind complement(2272..2296) /vntifkey="28" /label=JF16 /note="anti-sense ADH T primer" primer complement(2385..2402) /vntifkey="27" /label=CAS_02 /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s" primer_bind complement(2213..2230) /vntifkey="28" /label=pEG202R /note="reverse primer for ADH terminator region" old_sequence <9011..>49 /vntifkey="89" /label=pBr\ori /note="pBR backbone and ori" BASE COUNT 2900 a 2241 c 2277 g 2756 t ORIGIN 1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg 61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa 121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg 181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg 241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc 301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc 361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa 421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg 481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata 541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag 601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg 661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa 721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt 781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac 841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct 901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata 961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact 1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc 1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt 1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac 1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg 1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta 1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc 1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg 1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca 1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca 1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg 1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa 1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct 1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact 1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa 1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg 1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg 1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt 2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat 2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattgg cccccaagaa 2161 aaagagaaag gtggaattcc cggggatccg tcgacctgca gccaagctaa ttccgggcga 2221 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 2281 aattttaaag tgactcttag gttttaaaac gaaaattctt gttcttgagt aactctttcc 2341 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta 2401 ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg tagatatgct 2461 aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga ggacaacacc 2521 tgttgtaatc gttcttccac acggatcgat ccacaggacg ggtgtggtcg ccatgatcgc 2581 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc 2641 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag 2701 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag 2761 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 2821 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 2881 actaccgcat taaagctagc tttgaagaaa aatgcgcctt attcaatctt tgctataaaa 2941 aatggcccaa aatctcacat tggaagacat ttgatgacct catttctttc aatgaagggc 3001 ctaacggagt tgactaatgt tgtgggaaat tggagcgata agcgtgcttc tgccgtggcc 3061 aggacaacgt atactcatca gataacagca atacctgatc actacttcgc actagtttct 3121 cggtactatg catatgatcc aatatcaaag gaaatgatag cattgaagga tgagactaat 3181 ccaattgagg agtggcagca tatagaacag ctaaagggta gtgctgaagg aagcatacga 3241 taccccgcat ggaatgggat aatatcacag gaggtactag actacctttc atcctacata 3301 aatagacgca tataagtacg catttaagca taaacacgca ctatgccgtt cttctcatgt 3361 atatatatat acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc 3421 gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc 3481 cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg 3541 ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat 3601 attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt 3661 gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc 3721 gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta 3781 agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag 3841 tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa 3901 cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg 3961 gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa 4021 gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc 4081 ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag 4141 aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta 4201 gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg 4261 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt 4321 ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat 4381 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg 4441 catttttgtt ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt 4501 gcaagatgga aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata 4561 gatgcaatag tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact 4621 atatattatt atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga 4681 tgttcaaaat tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg 4741 atattaggct gtatctcctc aaagcgtatt cgaatatcat tgagaagctg cagcaggcgt 4801 gaagttagac gacaacttct ctctggaaac gcataccgat attcaggctg ctgcaaaggc 4861 acaggctagt gcccgtgcga gtgcatccgg taccacccca gatgctgtag tagcttctgg 4921 tagcactgca atgagccatg cttatcaaga aaacacaggt tttggtactc gtcccatata 4981 tcttgacatg caagccacta caccaacaga ccctagggtt ttggatacga tgttgaagtt 5041 ttatacggga ctttatggta atcctcattc caacactcac tcttacggtt gggaaacaaa 5101 tactgctgtg gaaaatgcta gagctcacgt agcaaagatg atcaatgccg accccaagga 5161 aataatattc acttcgggag cgaccgaatc taataatatg gttcttaagg gtgtcccaag 5221 attttataag aagactaaga aacacatcat caccactaga acggaacaca agtgtgtctt 5281 ggaagccgca cgggccatga tgaaggaggg atttgaagtc actttcctaa atgtggacga 5341 tcaaggtctt atcgatttga aggaattgga agatgccatt agaccagata cctgtctcgt 5401 ctctgtgatg gctgtcaata atgaaatcgg tgtcattcaa cctattaaag aaattggagc 5461 aatttgtaga aagaataaga tcctcgggga caccaaatat ggcgatctcg gccttttcgt 5521 ttcttggagc tgggacatgt ttgccatcga tccatctacc accagaacgg ccgttagatc 5581 tgctgccacc gttgtttcca ccgaagaaac caccgttgcc gtaaccacca cgacggttgt 5641 tgctaaagaa gctgccaccg ccacggccac cgttgtagcc gccgttgttg ttattgtagt 5701 tgctactgtt atttctggca cttcttggtt ttcctcttaa gtgaggagga acataaccat 5761 tctcgttgtt gtcgttgatg cttaaatttt gcacttgttc gctcagttca gccataatat 5821 gaaatgcttt tcttgttgtt cttacggaat accacttgcc acctatcacc acaactaact 5881 ttttcccgtt cctccatctc ttttatattt tttttctcga tcgagttcaa gagaaaaaaa 5941 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg 6001 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat 6061 aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat aatcggtgtc 6121 actacataag aacacctttg gtggagggaa catcgttggt accattgggc gaggtggctt 6181 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc 6241 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt tcaagaaaat 6301 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa 6361 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg 6421 caaatcctga tccaaacctt tttactccac gcgccagtag ggcctcttta aaagcttgac 6481 cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc 6541 actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata tggtccagaa 6601 accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct gctactgctt 6661 ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc tttaaagaga 6721 tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc tgctctgtca 6781 tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa gaaatcacat 6841 tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag 6901 tcatccgcta ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga 6961 gtgtactaga ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgtgttat 7021 gacttccctg actaatgccg tgttcaaacg atacctggca gtgactccta gcgctcacca 7081 agctcttaaa acgagaatta agaaaaagtc gtcatctttc gataagtttt tcccacagca 7141 aagcaatagt agaaaaacaa tgggaaacgt tgaatgaaga caaagcgtcg tggtttaaaa 7201 ggaaatacgc tcacgtacat gctagggaac aggaccgtgc agcggatcta atgaatccat 7261 ttgttagtta atagtttaaa tgtttttatc ggaagaggtt ttgtcatcac atcagcaatg 7321 ttcttcttgg tctcgatgta gtatacgtat aaattattac ctgatacttc atctctaagt 7381 ctcattgcct ttgtgccaaa aaatctgttt ctaaatttct cttcatttgt agacttaatt 7441 atactgatcg ttgatctact atcagtaagt aagcctttaa aaaaaaaaaa aaaaaaaaaa 7501 aaaaaaaaac ctgtaacaat agcaataccc caaataccta atgtagttcc agcaagcaag 7561 ctaaaaagta aagcaacaac ataactcacc cctgcatctg cagcttttgc ccgggcagcc 7621 tgctctgcct gtgttttctt taattgagca gtagaccatt tagcagttgc atgaatagct 7681 gcagcgtcac atcggataat aatgatggca gccattgtag aagtgccttt tgcatttcta 7741 gtctctttct cggtctagct agttttacta catcgcgaag atagaatctt agatcacact 7801 gcctttgctg agctggatca atagagtaac aaaagagtgg taaggcctcg ttaaaggaca 7861 aggacctgag cggaagtgta tcgtacagta gacggagtat actagtatag tctatagtcc 7921 gtggaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 7981 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt 8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 9301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 9841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 9901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc 9961 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 10021 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 10081 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 10141 atccgcttac agacaagctg tgaccgtctc cggg //